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9:57
$
jove.com
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
10.5K Views. University of Quebec in Montreal. This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.
10.7K views
Sep 20, 2016
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