In molecular biology, enzymes play critical roles in DNA replication, transcription, translation, PCR (polymerase chain reaction), cloning, and other molecular techniques.

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered ...

Molecular biology has transitioned to a new era where precise genetic manipulation plays a critical role in research, medicine, and biotechnology. Among the vital tools in this field are cloning ...

The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

Type I and type III restriction enzymes do not have practical value because they cannot produce specific cleavage fragments. Type II restriction enzymes have been widely used in DNA molecular cloning ...

An international team has identified a key enzyme in the reprogramming process that promotes malignant stem cell cloning and the growth of chronic myeloid leukemia (CML), a cancer of the blood and ...

Enzymes Involved in Molecular Cloning There are three types of enzymes used for cloning. These include restriction endonucleases, polymerases, nucleases, ligases, kinases, and phosphatases.